Protein Production in Eukaryotic Cells -- Production of Protein Therapeutics in the Quality by Design (QbD) Paradigm -- Characterization of Therapeutic Proteins -- Immunogenicity Lessons Learned from the Clinical Development of Vatreptacog Alfa, A Recombinant Activated Factor VII Analog, in Hemophilia with Inhibitors -- The Art of Gene Redesign and Recombinant Protein Production: Approaches and Perspectives.

Optimizing Lactic Acid Cheese Packaging Systems -- Evolutive Profiles of Caseins and Degraded Proteins in Industrial Cow’s Milk Curds -- The Production of Industrial Cow’s Milk Curds -- Chemical Correlations between Industrial Curds and Final Cheeses. Can Cheese Makers Standardize Productions?

Introduction -- Early Life 1918-1943 -- Protein Period 1943-1962 -- Origins of the Medical Research Council Laboratory of Molecular Biology.- RNA Period 1962-1971 -- DNA Period 1969-1983 -- Life outside the Lab -- The Man and His Legacy.

Fluorescence Sensing of Inorganic Phosphate and Pyrophosphate Using Small Molecular Sensors and Their Applications -- Metal Fluorides: Tools for Structural and Computational Analysis of Phosphoryl Transfer Enzymes -- Importance of Radioactive Labelling to Elucidate Inositol Polyphosphate Signalling -- Applications and Advantages of Stable Isotope Phosphate Labeling of RNA in Mass Spectrometry -- New Synthetic Methods for Phosphate Labeling -- Phosphate-Modified Nucleotides for Monitoring Enzyme Activity -- Chemical Approaches for Studying Labile Amino Acid Phosphorylation -- Applications of Phosphate Modification and Labeling to Study (m)RNA Caps.

Structural basis of permeation function of plant aquaporins -- Heteromerization of plant aquaporins -- Plant aquaporin trafficking -- Plant aquaporin post-translational regulation -- Plant aquaporins and cell elongation Aquaporins and root water uptake -- Aquaporins and leaf water relations -- Roles of aquaporins in stomata -- Aquaporins and abiotic stress -- Plant aquaporins and signaling -- Role of aquaporins in maintenance of xylem hydraulic capacity -- Plant aquaporins and CO2 -- NIP subfamily -- Plant aquaporins and metalloids -- Plant aquaporins and mycorrhizae: their regulation and involvement in plant physiology and performance.

Foreword -- Chap. 1 Anti-depressant functions of sigma receptor 1 -- Chap. 2 Parkinsonism -- Chap. 3 New ligands for sigma receptors and studies of ligand binding sites -- Chap. 4 σR1 and neurite elongation -- Chap. 5 Studies of σR1 in ALS, photaffinity probes and oxidative stress -- Chap. 6 σR1: Pharmacology and protective effects -- Chap. 7 Anti-stroke and anti-excitotoxicity -- Chap. 8 σR1 as a molecular chaperone -- Chap. 9 The potential role of σR1 as a neuroprotective -- Chap. 10 Studies of σR1 and effects on VGCC (calcium channels) -- Chap. 11 The role of σR1 in retinal neuroprotection -- Index.

Chapter 1. Protein folding in the cell – the role of molecular chaperones -- Chapter 2. Structure and function of the Hsp60 Chaperonins -- Chapter 3. Classical View on the Regulation of Heat-shock response -- Chapter 4. Recent Advances in the Regulation of Heat-shock Response- Chapter 5. Multiple Chaperonins in Bacteria -- Chapter 6. Multiple Chaperonins in Mycobacteria -- Chapter 7. Dynamic interplay of the Myxobacterial chaperonins -- Chapter 8. Division of Labour in Rhizobial Chaperonins -- Chapter 9. Cooperativity of archaeal and bacterial chaperonins -- Chapter 10. Evolution of multiple chaperonins.

Section I: Tyrosinemia Type 1: Heredity -- Section II:The Molecular Basis of HTI -- Section III: Pathology -- Section IV: Screening, Management and The Future.

Fibrous Protein Structures: Hierarchy, History and Heroes -- Coiled-coil design: updated and upgraded -- Functional and Structural Roles of Coiled Coils -- The Structure and Topology of α-Helical Coiled Coils -- Structural Transition of Trichocyte Keratin Intermediate Filaments During Development in the Hair Follicle -- Crystallographic studies of intermediate filament proteins -- Lessons from animal models of cytoplasmic intermediate filament proteins -- Filamentous Structure of Hard β-Keratins in the Epidermal Appendages of Birds and Reptiles -- Tropomyosin Structure, Function, and Interactions: A Dynamic Regulator -- Titin and Nebulin in Thick and Thin Filament Length Regulation -- Myosin and Actin Filaments in Muscle: Structures and Interactions -- Dystrophin and spectrin, two highly dissimilar sisters of the same family -- Fibrin Formation, Structure and Properties -- Fibrillar collagens -- Recombinant structural proteins and their use in future materials -- Properties of engineered and fabricated silks -- Biomaterials Made from Coiled Coil Peptides -- Bioengineered Collagens. .

Introduction -- Analysis of GAPDH enzyme activity: A quantitative and qualitative approach -- Analysis of GAPDH - Native protein -- Analysis of GAPDH under Denaturing Conditions -- Analysis of GAPDH and Protein Interaction -- Analysis of GAPDH and Nucleic acid Interaction -- Analysis of Subcellular and Extracellular GAPDH -- Analysis of GAPDH posttranslational modifications -- Analysis of GAPDH by Mass Spectrometry -- Purification of GAPDH.

Mobile Telephony EMFs Effects on Insect Ovarian Cells. The Necessity for Real Exposures Bioactivity Assessment. The key role of Polarization, and the “Ion Forced-Oscillation Mechanism”, Dimitris J. Panagopoulos -- Effect of Microwaves on DNA and Proteins, Takeo Yoshimura, Shuntaro Tsubaki, Satoshi Fujii, Masato Maitani, Eiichi Suzuki, and Yuji Wada -- Microwave effects on DNA, Jitendra Behari and Tanu Jindal -- Modified health effects of non-ionizing electromagnetic radiation combined with other agents reported in the biomedical literature, Dr. Ronald N. Kostoff -- Misleading Scientific Papers on Health Effects from Wireless Communication Devices, Luc Verschaeve -- Application of Microwave Irradiation to Bone Decalcification and its Effect on DNA Quality, Kazuhiko Imaizumi -- Ultra-High-Frequency Electromagnetic Radiation and Reactive Species in Mammals, Furtado-Filho, O.V. -- Lyse-It™: A Rapid Platform for Cellular Lysing and Tunable DNA/Protein Fragmentation, Chris D. Geddes et al -- Index.

RUNX in invertebrates -- Structure and biophysics of CBFb/RUNX and its transcription products -- Covalent modification of RUNX proteins: structure affects function -- The role of Runx1 in embryonic blood cell formation -- RUNX1 structure and function in blood cell development -- Roles of Runx2 in skeletal development -- Roles of Runx genes in nervous system development -- Runx family genes in tissue stem cell dynamics -- Roles of RUNX1 enhancer in normal hematopoiesis and leukemogenesis -- RUNX1-ETO leukemia -- Clinical relevance of RUNX1 and CBFB alterations in acute myeloid leukemia and other hematological disorders -- Mechanism of ETV6-RUNX1 leukemia -- ETV6-RUNX1+ acute lymphoblastic leukemia in identical twins -- Molecular basis and targeted inhibition of CBFb-SMMHC acute myeloid leukemia -- The RUNX genes as conditional oncogenes: insights from retroviral targeting and mouse models -- RUNX1 and CBFb mutations and activities of their wild-type alleles in AML -- Roles of RUNX in B cell immortalization -- Roles of RUNX in solid tumors -- RUNX3 and p53: How two tumor suppressors cooperate against oncogenic Ras -- Runx3 and cell fate decisions in pancreas cancer -- RUNX genes in breast cancer and the mammary lineage -- Runx3 in immunity, inflammation and cancer -- Roles of RUNX complexes in immune cell development -- CBFb and HIV infection -- Roles of RUNX in Hippo pathway signaling -- Roles of RUNX in hypoxia-induced responses and angiogenesis -- The emerging roles of RUNX transcription factors in Epithelial-Mesenchymal Transition -- Regulatory role of RUNX1 and RUNX3 in the maintenance of genomic integrity.

Overview of the diverse roles of bacterial and archaeal cytoskeletons -- E. coli cell cycle machinery -- Cell cycle machinery in Bacillus subtilis -- Cytoskeletal proteins in Caulobacter crescentus -- FtsZ constriction force – curved protofilaments bending membranes -- Intermediate filaments supporting cell shape and growth in bacteria -- Z-ring architecture and its control by MinCD co-polymers -- Bacterial actins -- Bacterial nucleoid occlusion -- Structure and dynamics of actin-like cytomotive filaments in plasmid segregation -- Tubulin-like proteins in prokaryotic DNA positioning -- The structure, function and roles of the archaeal ESCRT apparatus -- Archaeal actin-family filament systems -- The tubulin superfamily in archaea -- Reconstitution of protein dynamics involved in bacterial cell division.

I. RNA synthesis -- Systems approaches to genome occupancy and output of the archaeal transcription machinery -- Mechanisms of transcription regulation in archaea -- 2. RNAdegradingenzymes Archaeal Exosome -- RNA-degrading enzymes in Archaea -- 3.Translation, tRNA&rRNA processing -- Archaeal translation -- Life and Death of Ribosomes in Archaea -- Structure and Function of archaeal RNase P -- Complex Biosynthesis of N6-threonyl-carbamoyl adenosine - an anti-frameshift nucleoside -- 4.Non codingRNAs -- Evolution of C/D box sRNAs -- Computational Screens for C/D Box and H/ACA sRNAs, and Potential New Roles in Regulation -- Small non coding RNAs in methanogenic Archaea -- Small non coding RNAs in halophilic Archaea -- 5.CRISPR defense system -- CRISPR-Cas systems in Haloarchaea.

Preface -- Introduction to Sumoylation -- Part I Molecular Functions -- Roles of Sumoylation in mRNA Processing and Metabolsim -- SUMO and Chromatin Remodeling -- Functions of SUMO in the Maintenance of Genome Stability -- Regulation of Cellular Processes by SUMO: Understudied Topics -- The Molecular Interface Between The SUMO And Ubiquitin Systems -- . SUMO and Nucleocytoplasmic Transport -- SUMO Modification of Ion Channels -- The Roles of SUMO in Metabolic Regulation -- Part II Cell Growth Regulation -- The SUMO Pathway in Mitosis -- Wrestling with Chromosomes: The Roles of SUMO During Meiosis -- Sumoylation in Development and Differentiation -- The Role of Sumoylation in Senescence -- Regulation of Plant Cellular and Organismal Development by SUMO -- SUMO in Drosophila Development -- Part III Diseases -- SUMOylation: Implications for Neurodegenerative Diseases -- Sumoylation and Its Contribution to Cancer -- Sumoylation Modulates the Susceptibility to Type 1 Diabetes -- Sumoylation in Craniofacial Disorders -- Coordination of Cellular Localization-Dependent Effects of Sumoylation in Regulating Cardiovascular and Neurological Diseases -- Viral Interplay with the Host Sumoylation System -- SUMOylation as an Integral Mechanism in Bacterial Infection and Disease Pathogenesis -- Index.

1. Directed evolution for Opsin Engineering (Prof. Frances H. Arnold, Caltech, USA) -- 2. Directed evolution for enzyme enantio-selectivity (Prof. Manfred T. Reetz, Max-Planck-Institut für Kohlenforschung, Germany) -- 3. Directed evolution for fuels and chemicals (Prof. Huimin Zhao, University of Urbana-Champaign, Illinois, USA) -- 4. Directed evolution for cancer biopharmaceuticals (Prof. Dane Wittrup, MIT, USA) -- 5. Directed evolution of the ligninolytic consortium (Prof. Miguel Alcalde, Institute of Catalysis, CSIC, Madrid, Spain) -- 6. Directed evolution for engineering of metabolic pathways (Prof. Claudia Schmidt-Dannert, University of Minnesota, USA) -- 7. Directed evolution and metagenomics (Prof. Kenneth N. Timmis, Technische Universität Braunschweig, Germany) -- 8. Directed evolution and protein evolvability (Prof. Jesse Bloom, Fred Hutchinson Cancer Research Center, Seattle, USA) -- 9. Directed evolution for understanding natural evolution (Prof. Dan S. Tawfik, Weizmann Institute of Science, Rehovot, Israel) -- 10. Diversity methods for directed evolution (Prof. Ullrich Schwaneberg, RWTH-Aachen University, Germany) -- 11. Ancestral enzymes, evolution and resurrection (Prof. Jose Manuel Sanchez Ruiz, Universidad de Granada, Spain) -- 12. Directed evolution and computational protein algorithms (Prof. Victor Guallar, Barcelona Supercomputing Center, Spain).

Heat Shock Proteins and maternal contribution to oogenesis and early embryogenesis -- The unmysterious roles of HSP90: Ovarian biology and autoantibodies -- The role of heat shock factors in mammalian spermatogenesis -- Heat shock protein A2 (HSPA2): Regulatory roles in germ cell development and sperm function -- The potential functions of small heat shock proteins in the uterine musculature during pregnancy -- The role of HSP70 in the regulation of autophagy in gametogenesis, pregnancy and parturition -- Roles of Grp78 in female mammalian reproduction.

Introduction -- BMPs: Historic and Future Perspectives -- BMP – Biology -- BMPs in Development -- BMP signaling -- BMP Antagonists - Biology -- BMP and BMP Antagonists - Structure and Function -- BMPs in Orthopaedic Medicine: Promises and Challenges -- BMP6 and Bone Repair -- BMPs in Dental Medicine: Promises and Challenges -- BMPs in Spine -- BMPs in Articular Joints -- BMP Signaling & FOP -- BMP-7 Biology and its Therapeutic Utility for Chronic Kidney Diseases -- BMPs in Inflammation -- BMPs and Angiogenesis -- BMP Signaling in Iron Homeostasis -- BMP Signaling in Pulmonary Arterial Hypertension -- BMP Signaling and Cancer -- BMP Biology in Rare Diseases.

1.Introduction -- 2. Evolution of phycobiliproteins -- 3. Structural and functional significance -- 4.Gene manipulation and biosynthesis of phycobiliproteins -- 5. Stress response of phycobiliproteins -- 6. Advances in production technology -- 7. Advances and strategies of purification technology -- 8. Biotechnological applications -- 9. Role in therapeutic sciences -- 10. Future development and challenges.