Part 1. Introduction to the CRISPR Revolution.- 1. Viral Vectors, Engineered Cells and the CRISPR Revolution -- 2.  Combining Engineered Nucleases with Adeno-associated Viral Vectors for Therapeutic Gene Editing -- Part 2. CRISPR in Model Systems --  3. From Reductionism to Holism: Toward a More Complete View of Development through Genome Engineering -- 4. Genome Engineering in Mice -- 5. Genome Editing to Study Ca2+ Homeostasis in Zebrafish Cone Photoreceptors -- 6. From Prokaryotic Immune Systems to Plant Genome Editing Tools -- 7. Part 3. The Future of CRISPR -- 7. Using High-throughput Gene Editing to Identify Therapeutic Targets in the Noncoding Genome -- 8. CRISPR in the Retina: Evaluation of Future Potential -- 9. The Future of CRISPR Applications in the Lab, the Clinic and Society.